Phosphatase, Acid from sweet potato
SIGMA/P1435 - ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)
Synonym: (Orthophosphoric-
CAS Number: 9001-77-8
EC Number: 232-630-9
MDL Number: MFCD00131847
Product Type: Chemical
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| form | ammonium sulfate suspension |
| InChI | 1S/C6H10O2/c1-3-4-8-5-6(2 |
| InChI key | GZCWLCBFPRFLKL-UHFFFAOYSA |
| mol wt | 110 kDa |
| Quality Level | 300  |
| secondary activity | ≤9.9 units/mg protein ATPase |
| SMILES string | O(CC(O)C)CC#C |
| specific activity | ≥10.0 units/mg protein (modified Warburg-Christian) |
| storage temp. | 2-8°C |
| Application: | Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. |
| Application: | Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase. |
| Biochem/physiol Actions: | Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes. |
| General description: | Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C. |
| Physical form: | Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10.0 units/mg protein (modified Warburg-Christian); secondary activity: ≤9.9 units/mg protein ATPase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.3.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |