PCR 100 bp Low Ladder
SIGMA/P1473 - for electrophoresis of PCR fragments
Synonym: 100bp maker; 100bp marker for agarose gel electrophoresis; 100pb ladder for agarose gel electrophoresis; DNA ladder; DNA marker; gel ladder; gel marker
Product Type: Chemical
P1473 on a 2% agarose gel
| biological source | synthetic |
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 75 uses |
| Application: | PCR 100 bp Low Ladder has been used: • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis • to visualize DNA from enzyme digested PCR mixture • to estimate the molecular weight of the PCR product fragments |
| General description: | Sigma’s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel. |
| General description: | The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments. |
| Other Notes: | Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats. |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 2.0%. |
| Other Notes: | Sigma’s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41141827 |