Anti-Phosphatidylserine Receptor antibody produced in rabbit
SIGMA/P1495 - 1-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-PSR
MDL Number: MFCD04040521
Product Type: Chemical
Immunoblotting Lysates of HeLa (lanes 1-2), Jurkat (lanes 3-4) and Molt-4 (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-PS Receptor (Cat. No. P1495) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1,3,5. Antibody 1 μg/mL 2,4,6. Antibody 1 μg/mL + PS Receptor immunizing peptide (human, 363-381)
Immunoblotting Lysate of K562 cells was separated on SDS-PAGE, blotted with Anti-PS Receptor (Cat. No. P1495) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 1 μg/mL + PS Receptor immunizing peptide (human, 363-381)
Immunoblotting Lysate of A431 cells was separated on SDS-PAGE, blotted with Anti-PS Receptor (Cat. No. P1495) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 1 μg/mL + PS Receptor immunizing peptide (human, 363-381)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1-1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50-70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 1:1,000 using whole extracts of human HeLa or A549 cells | |
| western blot: 1:500 using whole extracts of mouse NIH3T3 cells | |
| UniProt accession no. | Q6NYC1  |
| Application: | Anti-Phosphatidylserine Receptor antibody is sutiable for use in immunoprecipitation and immunoblotting (~50-70 kDa) applications. |
| Biochem/physiol Actions: | Anti-Phosphatidylserine Receptor (PSR) recognizes human and mouse phosphatidylserine receptor (PSR). Detection of the phosphatidylserine receptor by immunoblotting is specifically inhibited by the immunizing peptide, a synthetic peptide corresponding to amino acid residues 363-381 of the human phosphatidylserine receptor, conjugated to maleimide-activated KLH as immunogen. |
| Biochem/physiol Actions: | Phosphatidylserine receptor (PSR) is very susceptible to cleavage by protease or elastase. PSR engagement plays an important role in macro-pinocytosis and uptake of tethered apoptotic cells. Cooperation with other receptors and their ligands may also be needed for ingestion of apoptotic cells. PSR is involved in suppression of inflammatory and immune responses. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Phosphatidylserine Receptor (PSR) is an evolutionarily conserved glycoprotein that is widely expressed in cells and tissues. It is very susceptible to cleavage by protease or elastase. PSR engagement plays an important role in macro-pinocytosis and uptake of tethered apoptotic cells. Cooperation with other receptors and their ligands may also be needed for ingestion of apoptotic cells. PSR is involved in suppression of inflammatory and immune responses. |
| General description: | Phosphatidylserine receptor belongs to the group of Jumonji domain containing protein 6 (JMJD6). It is known to be localized to the phagocytes. PSR is an evolutionarily conserved glycoprotein that is widely expressed in cells and tissues. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 363-381 of the human phosphatidylserine receptor conjugated to maleimide-activated KLH. The corresponding sequence in mouse differs by one amino acid. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mm sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |