Pyruvate Kinase from rabbit muscle
SIGMA/P1506 - Type II, ammonium sulfate suspension, 350-600 units/mg protein
Synonym: ATP:pyruvate 2-
CAS Number: 9001-59-6
MDL Number: MFCD00081946
Product Type: Chemical
| biological source | rabbit muscle |
| color | white |
| foreign activity | lactic dehydrogenase, creatine phosphokinase, and myokinase ≤0.01% |
| phosphoglucomutase ≤0.05% | |
| form | ammonium sulfate suspension |
| mol wt | 237 kDa |
| Quality Level | 200  |
| specific activity | 350-600 units/mg protein |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| technique(s) | ligand binding assay: suitable |
| type | Type II |
| Analysis Note: | Protein determined by biuret. |
| Application: | Pyruvate kinase from rabbit muscle has been used in: • a structural study to understand the reaction mechanism of the final step in glycolysis. • a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids. • enzyme assays. |
| Biochem/physiol Actions: | Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa. Isoelectric Point: 7.6 Optimal pH: ∼7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. |
| Biochem/physiol Actions: | Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn2+). In the glycolytic pathway, pyruvate kinase (PK) functions as a terminal enzyme, catalyzing the conversion of phosphoenolpyruvate to pyruvate and the synthesis of ATP through substrate-level phosphorylation. Tetrameric structures of PK are more active and have a high affinity for phosphoenolpyruvate (PEP), whereas dimeric structures are less active and have a low affinity for PEP. PK from rabbit muscle possesses positive kinetic cooperativity (Hill coefficient> 1.35) of the phosphoenol pyruvate and adenosine diphosphate(ADP) binding sites. |
| General description: | Research Area: Cell Signaling Pyruvate kinase from rabbit muscle catalyzes ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.Pyruvate kinase, an enzyme, is found in a tetrameric or a dimeric form. PKM1, PKM2, PKR, and PKL are the four mammalian pyruvate kinase isoforms. |
| Other Notes: | One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C. |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 solution, pH 6 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 350-600 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.1.40 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |