Thermolysin from Geobacillus stearothermophilus
SIGMA/P1512 - Type X, lyophilized powder, 30-350 units/mg protein (E1%/280 )
Synonym: Protease from Geobacillus stearothermophilus; Thermophilic-
CAS Number: 9073-78-3
EC Number: 232-973-4
MDL Number: MFCD00165549
Product Type: Chemical
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| biological source | Geobacillus stearothermophilus |
| form | lyophilized powder |
| mol wt | 34.6 kDa by amino acid sequence |
| purified by | crystallization |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | 30-350 units/mg protein (E |
| storage temp. | −20°C |
| type | Type X |
| Analysis Note: | Contains many extraneous enzymes. |
| Application: | Non-specific Protease Activity Assay Video |
| Application: | A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes. |
| Application: | Thermolysin has been shown to have a prosequeence that acts as an intramolecular chaperone in vivo. It has also been used in a study to investigate the effects of sodium chloride on thermal stability and catalytic activity. |
| Application: | Thermolysin is also commonly used for the commercial synthesis of N-(benzyloxycarbonyl)-L-a |
| General description: | Thermolysin is a protease that has specificity different from other proteases available for sequence investigations. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 250 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Physical form: | lyophilized powder containing calcium and sodium acetate buffer salts |
| Preparation Note: | The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautionary statements | P261 - P280 - P305 + P351 + P338 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42/43 |
| Safety Statements | 22-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 30-350 units/mg protein (E |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.27 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |