o-Phenylenediamine dihydrochloride
SIGMA/P1526 - peroxidase substrate
Synonym: OPD
CAS Number: 615-28-1
Empirical Formula (Hill Notation): C6H8N2 · 2HCl
Molecular Weight: 181.06
EC Number: 210-418-7
MDL Number: MFCD00012966
Linear Formula: C6H4(NH2)2·2HCl
Product Type: Chemical
o-Phenylenediamine (OPD) is a chromogenic substrate suitable for use in ELISA procedures which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3 M H2SO4, and read at 492 nm.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1526-1KG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1526-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1526-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1526-50G
| color | white to off-white |
| form | solid |
| InChI | 1S/C6H8N2.2ClH/c7-5-3-1-2 |
| InChI key | RIIWUGSYXOBDMC-UHFFFAOYSA |
| mp | 258 °C (dec.) (lit.) |
| Quality Level | 300  |
| SMILES string | Cl[H].Cl[H].Nc1ccccc1N |
| storage temp. | −20°C |
| Application: | o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures. |
| Disclaimer: | May darken in storage. |
| General description: | o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm. |
| Packaging: | 1 kg in glass bottle |
| Packaging: | 10, 25, 50, 100 g in glass bottle |
| Symbol |    GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 - H312 + H332 - H317 - H319 - H341 - H351 - H410 |
| Precautionary statements | P201 - P273 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P312 |
| Hazard Codes | T,N |
| Risk Statements | 20/21-25-36-40-43-50/53-68 |
| Safety Statements | 28-36/37-45-60-61 |
| RIDADR | UN 1673 6.1 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 258 °C (dec.) (lit.) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |