Polyinosinic–polycytidylic acid sodium salt

SIGMA/P1530 - TLR ligand tested

Synonym: Poly (I:C); Poly(I) • Poly(C)

CAS Number: 42424-50-0
MDL Number: MFCD00131984
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P1530-25MG	25 mg	$217.00 1/EA	Add To Favorites
45-P1530-100MG	100 mg	$649.00 1/EA	Add To Favorites

Polyinosinic–Polycytidylic acid sodium salt (Poly I:C) (Cat. No. P1530) induces proinflammatory cytokines through TLR3-TRIF complex.Cells (macrophage cells, WT and macrophages deficient in TRIF adaptor protein, TRIF KO) were plated (8×10⁴/well) and incubated for 4 hours. Poly I:C was then added at the indicated concentrations. Twenty hours following Poly I:C addition supernatants were tested for TNFα concentration using quantitative sandwich ELISA. The assay was performed in triplicates and mean values ±SD are shown. In collaboration with Gabriel Nussbaum, Faculty of Dental Medicine, The Hebrew University of Jerusalem.

Polyinosinic–polycytidylic acid sodium salt unit structure.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1530-100MG

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (less than 1% free nucleotides, TLC)
form	lyophilized powder
InChI	1S/C10H13N4O8P.C9H14N3O8P.Na/c15-6-4(1-21-23(18,19)20)22-10(7(6)16)14-3-13-5-8(14)11-2-12-9(5)17;10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18;/h2-4,6-7,10,15-16H,1H2,(H,11,12,17)(H2,18,19,20);1-2,4,6-8,13-14H,3H2,(H2,10,11,15)(H2,16,17,18);/t
InChI key	GETQKWBYQAUFQM-VRQAYDGLSA-N
Quality Level	300
SMILES string	[Na].[P](=O)(OC[C@H]3O[C@H]([C@@H]([C@@H]3O)O)[n]4c5ncnc(c5nc4)O)(O)O.[P](=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)N2C=CC(=NC2=O)N)(O)O
solubility	H₂O: 10 mg/mL
storage condition	desiccated
storage temp.	−20°C

Application:	TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.
Biochem/physiol Actions:	Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Other Notes:	Double-stranded homopolymer.
Packaging:	25, 100 mg in glass bottle
Preparation Note:	The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (less than 1% free nucleotides, TLC)
Storage Temp.	−20°C
UNSPSC	41106305

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