Anti-PRMT1 antibody, Mouse monoclonal
SIGMA/P1620 - clone PRMT1-171, purified from hybridoma cell culture
Synonym: Anti-
MDL Number: MFCD04975120
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171 (Cat. No. P1620). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PRMT1-171, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~42 kDa |
| shipped in | dry ice |
| species reactivity | mouse, chicken, bovine, human, canine, hamster, monkey, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 2 μg/mL using whole cell extract of 293T cells | |
| UniProt accession no. | Q99873   |
| Application: | Anti-PRMT1 antibody, Mouse monoclonal has been used for Immunofluorescence and Western Blotting. |
| Biochem/physiol Actions: | Does not cross-react with PRMT2, 3, 4, and 5. |
| Biochem/physiol Actions: | Protein arginine methyltransferase 1 (PRMT1) is crucial for the formation of asymmetric dimethylarginine. It can methylate Twist-1, a transcription factor. The protein also methylates histones and thus modulates transcription and protein activity. PRMT1 is involved in epithelial-mesenchymal transition and may have a role in cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein arginine methyltransferase 1 (PRMT1) gene is localized on human chromosome 19q13.33. It is part of the type I PRMT family. |
| Immunogen: | full length PRMT1. Does not cross-react with PRMT2, 3, 4, and 5. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 C for up to one month. For prolonged storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| Safety Statements | 36 |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |