Peroxidase, Maleimide Activated from horseradish
SIGMA/P1709 - lyophilized powder, >200 units/mg protein
Synonym: Maleimide activated HRP
MDL Number: MFCD00284131
Product Type: Chemical
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| biological source | horseradish |
| composition | Protein, ~50% E |
| extent of labeling | 1-3 mol maleimide per mol protein |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | >200 units/mg protein |
| storage temp. | −20°C |
| Application: | For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent. Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. |
| Application: | To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification. |
| Biochem/physiol Actions: | Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc. |
| General description: | Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms. |
| Other Notes: | One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C. |
| Other Notes: | Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester. |
| Physical form: | Lyophilized powder containing trehalose and sodium citrate |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >200 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |