Protease Inhibitor Cocktail
SIGMA/P1860 - for use in tissue culture media, DMSO solution
Synonym: Protease Inhibitor Solution; protease inhibitor
EC Number: 200-664-3
MDL Number: MFCD00677817
Product Type: Chemical
Suitability test for Protease Inhibitor Cocktail Inhibition of trypsin, OD/time
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1860-1ML
| form | DMSO solution |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | Protease Inhibitor Cocktail has been used: • as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS) • to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes • in trypsin solution for trypsin-inhibited studies • as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot |
| Application: | This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases. |
| Biochem/physiol Actions: | Inhibits serine, cysteine, aspartic proteases, and aminopeptidases |
| Biochem/physiol Actions: | Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue. |
| Disclaimer: | After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension. |
| Features and Benefits: | • Broad specificity • Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension. • Contains no metal chelators: Ensures compatibility with downstream applications • Convenient packaging: 1 mL in glass bottle • Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins. |
| General description: | Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure: A431, CHO, COS, HepG2, and HeLa adherent cell lines Jurkat and HL-60 cell lines grown in suspension |
| General description: | The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media. |
| Other Notes: | Aprotinin Bestatin E-64 Leupeptin Pepstatin A |
| Other Notes: | For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in DMSO (D 2650, Hybri-Max). |
| Preparation Note: | Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins. |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |