Plasmin from human plasma
SIGMA/P1867 - lyophilized powder, ≥2.0 units/mg protein
Synonym: Fibrinolysin
CAS Number: 9001-90-5
EC Number: 232-640-3
MDL Number: MFCD00131921
Product Type: Chemical
Native-intact human plasminogen is a 291 amino acid glycoprotein with as many as 24 disulfide bonds. Plasminogen contains a single N-linked sialylated biantennary glycan. The two O-glycans possess a Gal β-1-3GalNAc core which is α-2-3 sialylated at the terminal Gal. An additional disialylated form has a second sialic acid residue with an α-2-6 linkage to GalNAc. Mono- and disialylated forms occur at a molar ratio of 80:20 in human plasminogen.
Plasmin is converted to plasminogen by cleavage between Arg561 and Val562. The resulting activated plasmin consists of two disulfide-linked polypeptide chains. The plasmin heavy chain (MW 60 kDa) is derived from the amino terminal region of plasminogen. The light chain originates from the carboxyl-terminus of plasminogen. In vivo, the MW of the heavy chain can vary from 63 kDa to 12 kDa depending on the extent of proteolysis to the plasminogen from which it is derived.
| application(s) | diagnostic assay manufacturing |
| form | lyophilized powder |
| impurities | HIV, Hepatitis B and Hepatitis C, tested negative |
| Quality Level | 200  |
| specific activity | ≥2.0 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00747  |
| Application: | A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin. |
| Biochem/physiol Actions: | It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells. |
| Biochem/physiol Actions: | Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products. pH Optimum: 8.5 pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity Temperature Optimum: 37 °C with rapid inactivation at 56 °C |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes. |
| Other Notes: | One unit will produce one μmole of p-Nitroanilide from |
| Packaging: | Package size based on protein content |
| Physical form: | Lyophilized powder containing sodium phosphate and Trehalose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.7 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |