Anti-Phosphotyrosine antibody, Mouse monoclonal
SIGMA/P1869 - clone pT-154, purified from hybridoma cell culture
Synonym: Phospho-Tyr; Phospho-tyrosine; p-Tyr
MDL Number: MFCD00164715
Product Type: Chemical
Western Blotting Whole extract of A431 cells stimulated by EGF were separated on SDS-PAGE and probed with Anti-Phosphotyrosine antibody, Mouse monoclonal, Clone: pT-154 (Cat. No. P1869). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 2 μg/mL antibody 2. 4 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | pT-154, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: suitable |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF |
| Application: | Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in: • enzyme linked immunosorbent assay (ELISA) • western blot • immunohistochemistry |
| Biochem/physiol Actions: | Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP. |
| Biochem/physiol Actions: | Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain. |
| Immunogen: | tyrosine phosphorylated peptide. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |