Anti-Perilipin A/B antibody produced in rabbit
SIGMA/P1873 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FPLD4; Anti-PERI; Anti-PLIN
MDL Number: MFCD07370429
Product Type: Chemical
Immunofluorescence 3T3-L1 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-Perilipin A/B antibody produced in Rabbit (Cat. No. P1873). The antibody was developed using 1:200 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~62 kDa (also band at 46 kDa) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using differentiated mouse NIH3T3-L1 cells |
| western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of differentiated mouse NIH3T3-L1 cells |
| Application: | Anti-Perilipin A/B antibody produced in rabbit has been used in immunoblotting and immunofluorescence staining. |
| Application: | Rabbit polyclonal anti-Perilipin A/B antibody is used to tag Perilipin A/B for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Perilipin A/B in the regulation of triacylglycerol storage in adipocytes. |
| Biochem/physiol Actions: | Perilipin is a gatekeeper protein that is involved in regulating triacylglycerol storage in adipocyte through the suppression of basal lipolysis apparently through protecting triacylglycerol against hydrolysis. Perilipin also enhances cAMP-dependent protein kinase (PKA)-stimulated lipolysis by hormone-sensitive lipase (HSL) and non-HSLs. Perilipin knockout mice exhibit reduced adipose tissue mass and resistance to diet induced obesity. Their lipid storage droplets are coated with adipose differentiation-related protein (ADRP, adipophilin), that is devoid of phosphorylation by PKA. |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Perilipin A/B antibody recognizes mouse Perilipin A/B by immunoblotting (46 kDa and ~62 kDa) and indirect immunofluorescence. Detection of the perilipin A and B bands by immunoblotting is specifically inhibited with the immunizing peptide. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Perilipin is an intracellular neutral lipid storage droplet surface protein in white and brown fat adipocytes. It is also found in lower quantity coating droplets in steroidogenic-cells of the adrenal cortex, ovaries, and testicular Leydig cells, on the surface of smaller droplets containing cholesteryl esters. |
| Immunogen: | synthetic peptide corresponding amino acid residues 6-17 of mouse and rat perilipin A/B with C-terminal added cysteine, conjugated to KLH. The corresponding human sequence differs by two residues. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |