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Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

SIGMA/P1944 - Molecular Biology

Synonym: phenol chloroform RNA extraction

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-P1944-100ML 100 mL
 $89.90
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45-P1944-400ML 400 mL
 $273.00
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application(s) life science and biopharma
assay ≥99.0%
form liquid
grade Molecular Biology
pH 4.3-4.7
Quality Level 200 
storage temp. 2-8°C
Application: Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used: • for the extraction and purification of RNA.
• to isolate RNA from Arabidopsis siliques.
• to extract proteins and DNA from polysomal and non-polysomal fractions.
• in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.
General description: Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.
Packaging: 100, 400 mL in glass bottle
Purity ≥99.0%
Storage Temp. 2-8°C
UNSPSC 12190000

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