Anti-Podoplanin (HG-19) antibody produced in rabbit
SIGMA/P1995 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD05665247
Product Type: Chemical
Immunohistochemistry Frozen sections acetone fixed Rat kidney tissue sections were stained with 20 μg/mL Anti-Podoplanin (HG-19) antibody produced in Rabbit (Cat. No. P1995), followed by 1:50 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of Rat lung were separated on SDS-PAGE and probed with Anti-Podoplanin (HG-19) antibody produced in Rabbit (Cat. No. P1995). The antibody was developed using 1:20,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 5 μg/mL antibody 2. 2.5 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~38 kDa |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections |
| western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung | |
| UniProt accession no. | Q64294   |
| Application: | Anti-Podoplanin (HG-19) antibody produced in rabbit has been used in immunostaining, immunoblotting and immunofluorescence staining. |
| Biochem/physiol Actions: | Podoplanin expression is regulated by the lymphatic-specific homeobox gene Prox-1. Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It has a critical regulatory role in the formation of the lymphatic vasculature. Podoplanin does not seem to be upregulated in tumor angiogenesis. It is detectable in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. |
| Biochem/physiol Actions: | Podoplanin is a marker of lymphatic endothelial cells. It may have a role in tumor cell invasion and migration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Podoplanin is part of the type-1 transmembrane sialomucin-like glycoprotein family. The gene encoding this 43kDa protein is localized on rat chromosome 5. |
| General description: | Podoplanin is present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is also named E11 antigen and has been described to be present in normal, inflammatory, and neoplastic lymphatic capillary endothelium. Podoplanin is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells. It is composed of heavily glycosylated extracellular domain and a short intracytoplasmic tail. |
| Immunogen: | synthetic peptide of rat podoplanin (amino acids 111-129) with N-terminal cysteine, conjugated to KLH. The sequence in mouse differs by one residue. |
| Packaging: | 200 μL in poly bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |