Peroxidase from horseradish
SIGMA/P2088 - Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)
Synonym: Donor:hydrogen-
CAS Number: 9003-99-0
EC Number: 232-668-6
MDL Number: MFCD00071339
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P2088-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P2088-5KU
| absorbance ratio | RZ 2.6-3.4 |
| biological source | horseradish |
| form | essentially salt-free, lyophilized powder |
| InChI | 1S/H2O3/c1-3-2/h1-2H |
| InChI key | JSPLKZUTYZBBKA-UHFFFAOYSA |
| mol wt | ~44 kDa |
| Quality Level | 300  |
| SMILES string | [O+H2]O[O-] |
| specific activity | 200-300 units/mg solid (using pyrogallol) |
| storage temp. | 2-8°C |
| Analysis Note: | The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. |
| Application: | Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism. It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme. |
| Biochem/physiol Actions: | Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol. |
| Disclaimer: | Maintains activity at low pH and higher temperature. |
| General description: | Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. |
| Other Notes: | One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C. |
| Other Notes: | View more information on peroxidase at www.sigma-aldrich.com/enz. |
| Packaging: | 5000, 10000 units in glass bottle |
| Preparation Note: | Stabilized by chemical protection of the primary amines. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: 200-300 units/mg solid (using pyrogallol) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |