Monoclonal Anti-p27Kip1 antibody produced in mouse
SIGMA/P2092 - clone DCS-72, ascites fluid
MDL Number: MFCD00284080
Product Type: Chemical
Immunoblotting Whole extract of NIH/3T3 cells was separated on SDS-PAGE, blotted with 1:2,000 Monoclonal Anti-p27Kip1 Clone: DCS-72 (Cat. No. P2092) and developed using Goat Anti-Mouse IgG-ALP (Cat. No. A1293) and a NBT/BCIP (Cat. No. B5655) substrate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-72, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, monkey, canine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 1:200 using extract of cultured mouse fibroblast line NIH3T3 | |
| UniProt accession no. | P46527  |
| Application: | Monoclonal Anti-p27Kip1 antibody produced in mouse has been used in immunoblotting, immunofluorescence, immunohistochemistry, immunoprecipitation and immunocytochemistry. |
| Biochem/physiol Actions: | p27, the product of the kinase inhibition protein1 (kip1) gene, is an inducible inhibitor of cyclin dependent kinase activity. p27Kip1 interacts strongly with D-type cyclins complexed with CDK4 and more weakly with cyclin E and CDK2 complexes. Regulation by p27Kip1 may be an essential step in the pathway that links mitogenic signals to cell cycle progression and may be a key molecular event in the physiological process of cell cycle commitment or passage through the restriction point. Thus, the amount of p27Kip1 increases in quiescent cells and rapidly decreases after stimulation with specific mitogens. Constitutive expression of p27Kip1 in cultured cells causes cell cycle arrest in G1. |
| Biochem/physiol Actions: | Using immunoblotting, some preparations may exhibit higher and lower molecular weight bands. which may represent a polyubiquitinated species and breakdown products of p27Kip1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti p27Kip1 (mouse IgG1 isotype) is derived from the DCS-72 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant p27Kip1 protein of rodent origin. |
| Immunogen: | recombinant rodent p27Kip1 protein. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |