10X PCR Buffer
SIGMA/P2192 - Optimized for routine PCR with MgCl2 included
Synonym: PCR Buffer Solution; PCR Reaction Buffer
Product Type: Chemical
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| application(s) | agriculture |
| color | colorless |
| foreign activity | DNase, RNase, none detected |
| form | liquid |
| packaging | vial of 1.5 mL |
| Quality Level | 300  |
| storage temp. | −20°C |
| Application: | 10X PCR Buffer has been used as a component of the PCR mixture:• for the amplification of viral DNA • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp. |
| Features and Benefits: | • Standalone buffer • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312) |
| Legal Information: | JumpStart is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 41106306 |