Protein C from human plasma
SIGMA/P2200 - Activated, lyophilized powder, ≥90% (SDS-PAGE)
Synonym: Activated Protein C
CAS Number: 42617-41-4
MDL Number: MFCD00165567
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | human plasma |
| form | lyophilized powder |
| mol wt | heavy chain 41 kDa |
| light chain 21 kDa | |
| Quality Level | 200  |
| solubility | H2O: 1 mg/mL |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P04070  |
| Application: | Protein C from human plasma has been used for pre-treatment of endothelial cells prior to antibody-inhibition assay. It has also been used in activated protein C (APC) assay to determine its inhibitory effect on copper. |
| Biochem/physiol Actions: | In addition, activated protein C has been shown to inhibit TNF-α induced expression of the inflammatory proteins VCAM, ICAM-I, and Ilk-8 in endothelial cells. |
| Biochem/physiol Actions: | Protein C is a plasma, vitamin κ-dependent zymogen of a serine protease that can inhibit blood coagulation by inhibiting thrombin formation, selectively inactivating Factors Va and VIIIa. |
| Biochem/physiol Actions: | Protein C is a plasma, vitamin κ-dependent zymogen of a serine protease that can inhibit blood coagulation by inhibiting thrombin formation, selectively inactivating Factors Va and VIIIa. The Protein C anticoagulant pathway is triggered when thrombin binds to the endothelial cell proteoglycan, thrombomodulin. This complex, which cannot clot blood, is a potent activator of the protein C zymogen. Activation involves the release of a dodecapeptide from the N-terminal domain of the heavy chain. The activated Protein C (APC) then binds to protein S on cell surfaces and inactivates the coagulation factors Va and VIIIa by proteolysis. APC has also been shown to bind to receptors on the endothelium of large blood vessels. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Protein C from human plasma is encoded by the gene PROC. In human chromosome, the gene is localised on chromosome 2q14. Activated protein C (APC) cleaves protease activated receptor 1 (PAR1) resulting in cytoprotective and anti-inflammatory effects. Clinical trials with APC implicates its use in treating severe early onset preeclampsia in pregnant women and prolongs pregnancy and helps in perinatal outcomes. |
| Physical form: | Lyophilized powder from 20 mM Tris-HCl, pH 7.4, containing 0.1 M NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.69 ( BRENDA | IUBMB ) |
| UNSPSC | 12352202 |