10X PCR Buffer without MgCl2
SIGMA/P2317 - Optimized for routine PCR without MgCl2
Synonym: Magnesium-free PCR buffer
Product Type: Chemical
Representative packaging
| application(s) | agriculture |
| color | colorless |
| form | liquid |
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| Application: | 10× PCR Buffer without MgCl2 has been used with Sigma′s PCR enzymes. |
| Application: | 10X PCR Buffer without MgCl2 has been used as a component of polymerase chain reaction (PCR) amplification mixture for the mycotoxigenic mould DNA, parasite DNA and Fusarium oxysporum DNA amplification.It has also been used as a component of the PCR mix for the amplification of nuclear ribosomal internal transcribed spacer (ITS2) and the partial ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit rbcl gene for molecular and morphological characterization of Ulva sp from the Persian Gulf. |
| Features and Benefits: | • This product is tested for the absence of DNase and RNase. • Suitable for use with magnesium chloride. |
| General description: | 10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM each, λ DNA template at 1ng/100μL, and Taq DNA polymerase at 2.5 units/100μL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106306 |