Anti-Pseudomonas Exotoxin A antibody produced in rabbit
SIGMA/P2318 - whole antiserum
Synonym: Anti-ETA
MDL Number: MFCD00162779
Product Type: Chemical
ELISA Anti-Pseudomonas Exotoxin A antibody produced in Rabbit (Cat. No. P2318), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919), was serially diluted to detect Horsedise Peroxidase by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | (Pseudomonas aeruginosa) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:20,000 |
| indirect ELISA: 1:250,000 |
| Application: | Anti-Pseudomonas Exotoxin A antibody has been used in in vitro serum stability of fusion toxins and binding of targeted toxins. |
| Application: | Anti-Pseudomonas exotoxin A antibody produced in rabbit has been used: • in the in vitro analysis of the stability of fusion toxins in serum • to construct an epidermal growth factor receptor (EGFR)-targeted toxin to study its toxicity on Swiss mouse embryo NIH-3T3 cells transfected with human EGFR • in the determination of the in vivo half-life of immunotoxins in serum |
| Biochem/physiol Actions: | Pseudomonas exotoxin A is secreted by Pseudomonas aeruginosa and has potent cytotoxic effects. Exotoxin A enters the cells by receptor-mediated endocytosis, translocates to the cytoplasm and inhibits protein synthesis by ADP-ribosylating factor 2. Chimeric exotoxin A has applications in gene treatment of various diseases and probable treatment for cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Reacts against Pseudomonas exotoxin A, but shows no reaction against Staphylococcal enterotoxin A, Cholera toxin, and Staphylococcal enterotoxin B. The product has not been tested for neutralization potency against active Pseudomonas exotoxin A. |
| Immunogen: | exotoxin A from Pseudomonas aeruginosa. |
| Physical form: | This product is supplied as a liquid containing 15 mM sodium azide as preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |