Monoclonal Anti-PSD95 antibody produced in mouse
SIGMA/P246 - clone 7E3-1B8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MRD62; Anti-PSD95; Anti-SAP-90; Anti-SAP90
MDL Number: MFCD01324382
Product Type: Chemical
Immunofluorescence : SCK-CEN Mouse cortex primary neurons cultured for 10 days were stained for PSD95, Β-Tubulin III and nuclei. For PSD95 Monoclonal Anti-PSD95 antibody produced in Mouse (Cat. No. P246) and Anti-Mouse IgG (whole molecule)–FITC antibody produced in Goat (Cat. No. F2012) (green) were used. For Β-Tubulin III Anti-Β-Tubulin III antibody produced in Rabbit (Cat. No. T2200) and Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in Goat (Cat. No. T6778) (red). Nuclei were stained with Hoechst B2883 (blue/purple). Submitted by Dr G. Pani, Studiecentrum voor Kernenergie-Centre d′Etude d′Energie Nucleaire
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7E3-1B8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 95 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:500 |
| immunoprecipitation (IP): suitable | |
| western blot: 1:2,000 | |
| UniProt accession no. | P31016  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Immunofluorescence (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Postsynaptic density 95 (PSD-95) plays a major role in bidirectional synaptic plasticity, an important process, essential for learning and memory. It exhibits increased dynamics upon induction of plasticity. |
| Biochem/physiol Actions: | Reacts with both the recombinant and native rat PSD95. By immunofluorescence with rat hippocampal cells, a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with its proposed role in receptor clustering is observed. Fixation with cold methanol is recommended. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DLG4 (discs large MAGUK scaffold protein 4) gene codes for the postsynaptic density-95 (PSD95) protein. Postsynaptic density 95 (PSD-95), a synaptic scaffolding protein is also a membrane-associated guanylate kinase (MAGUK) family protein. It has three PDZ domains on the N terminus followed by a Src homology 3 (SH3) domain and a guanylate kinase (GK) domain. This gene is located on human chromosome 17p13.1. |
| Immunogen: | recombinant rat PSD95 (post synaptic density 95 kDa). |
| Physical form: | Solution in phosphate buffered saline containing 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |