Protein A–Agarose
SIGMA/P2545 - saline suspension
Synonym: Protein A resin
MDL Number: MFCD00165563
Product Type: Chemical
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| biological source | Staphylococcus aureus |
| capacity | ≥15 mg/mL binding capacity (human IgG) |
| extent of labeling | ~2 mg per mL |
| form | saline suspension |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| matrix spacer | 1 atom |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer. |
| Application: | Protein A−Agarose has been used in the immunoprecipitation • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells • of prolyl hydroxylase domain 2 N27 cells • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells |
| Biochem/physiol Actions: | Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications. |
| General description: | Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains. |
| Physical form: | Suspension in 0.5 M NaCl containing preservative |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |