Phosphomannose Isomerase from Escherichia coli
SIGMA/P2621 - recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein
Synonym: D-Mannose-6-phosphate ketol-isomerase; Mannose Phosphate Isomerase; PMI
MDL Number: MFCD00131899
Product Type: Chemical
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| form | ammonium sulfate suspension |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥50 units/mg protein |
| storage temp. | 2-8°C |
| Application: | PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD). |
| Biochem/physiol Actions: | Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation. |
| Other Notes: | One unit will convert 1.0 μmole of |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Physical form: | Supplied as a suspension in 3.2 M ammonium sulfate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 5.3.1.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |