Protein Kinase A Catalytic Subunit from bovine heart
SIGMA/P2645 - ≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
Synonym: PKA
MDL Number: MFCD00132114
Product Type: Chemical
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| form | lyophilized (white powder to sticky mass to hard pellet) |
| mol wt | 40,862 Da |
| Quality Level | 200  |
| specific activity | ≥9 units/μg protein (cyclic-AMP is not required for this activity) |
| storage temp. | −20°C |
| Application: | Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used- • to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels • in in Vitro PKA pPhosphorylation assay • in Vitro affinity binding assays • to study effects of PKA on inspiratory drive currents in functionally active motorneurons |
| Biochem/physiol Actions: | Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways. |
| Biochem/physiol Actions: | Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit. |
| Disclaimer: | Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before. |
| General description: | Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da. |
| Other Notes: | Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP. |
| Packaging: | Package size based on phosphorylating units |
| Physical form: | Lyophilized powder with sucrose and phosphate buffer salts as stabilizer. |
| Preparation Note: | Prepared from protein kinase A (P 5511) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥9 units/μg protein (cyclic-AMP is not required for this activity) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.11.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |