Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse
SIGMA/P2732 - clone 6H11, tissue culture supernatant
Synonym: Anti-BCDS2; Anti-CAS; Anti-CTNND; Anti-P120CAS; Anti-P120CTN; Anti-p120; Anti-p120(CAS); Anti-p120(CTN)
MDL Number: MFCD02097107
Product Type: Chemical
Immunofluorescence MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:100 Monoclonal Anti-p120ctn (Catenin-related) antibody produced in Mouse, Clone: 6H11 (Cat. No. P2732). The antibody was developed using 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract of cultured Chicken fibroblasts cells were separated on SDS-PAGE and probed with Monoclonal Anti-p120ctn (Catenin-related) antibody produced in Mouse, Clone: 6H11 (Cat. No. P2732). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:20,000 antibody 2. 1:10,000 antibody 3. Negative Control
| antibody form | tissue culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6H11, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, canine, chicken, mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:100 using cultured Madin Darby canine kidney (MDCK) epithelial cells | |
| western blot: 1:10,000 using whole cell extract of cultured chicken fibroblasts | |
| UniProt accession no. | O60716  |
| Application: | Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse has been used in: • immunoblotting • enzyme linked immunosorbent assay (ELISA), • immunocytochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Reacts specifically with the N-terminal region of p120ctn and recognizes isoforms 1 and 2, but not isoforms 3 or 4. In immunoblotting, the antibody recognizes a 120 kDa band, and an additional very weak doublet at 200 kDa. |
| Biochem/physiol Actions: | The 120 kD catenin-related protein p120ctn (also known as catenin p120ctn, p120cas and pp120 src substrate), was originally described as a prominent substrate for the sarcoma (Src) oncoprotein and for a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF), and colony stimulating factor-1 (CSF1). Like β-catenin and plakoglobin, it binds directly to the cytoplasmic domain of epithelial (E)-cadherin via the ARM domain and co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. However, unlike β-catenin and plakoglobin, p120ctn does not appear to bind α-catenin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-p120ctn (Catenin-related) (mouse IgG1 isotype) is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine p120ctn. Catenin δ-1 (p120ctn) contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and plakoglobin. At least six alternatively spliced p120ctn isoforms are generated and differentially expressed in different cell types. |
| Immunogen: | recombinant N-terminal fragment of murine p120ctn. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |