Anti-p300/CBP antibody, Mouse monoclonal
SIGMA/P2859 - clone NM11, purified from hybridoma cell culture
Synonym: Anti-CREB Binding Protein
MDL Number: MFCD02097119
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Monoclonal Anti-p300/CBP, Clone: NM11 (Cat. No. P2859). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Western Blotting Whole extract of HEK-293T cells were separated on SDS-PAGE and probed with Monoclonal Anti-p300/CBP antibody produced in Mouse, Clone: NM11 (Cat. No. P2859). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 10 μg/mL antibody 2. 20 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NM11, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 300 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human, mink, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 10-20 μg/mL using human 293 embryonal kidney cells | |
| UniProt accession no. | Q92831  |
| Biochem/physiol Actions: | p300/CBP are capable of binding to a variety of transcriptional activator and regulatory molecules, including p53 and nuclear hormone receptors. The complexity of these p300/CBP cellular associations suggests that both proteins play a central role in the coordination of gene expression during cell growth and differentiation. KAT2B (lysine acetyltransferase 2B) possess histone acetyltransferase (HAT) activity and is thus known to regulate transcription process. Acetylation also influences the chromatin structure. Lysine acetylation significantly contributes to protein modification. It is essential for establishing protein function by altering its structure, activity and molecular interaction. |
| Biochem/physiol Actions: | Reacts specifically with both p300 and CBP but not with the related p270 molecule. The epitope recognized by the antibody resides within the 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of the antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-p300/CBP (mouse IgG1 isotype) is derived from the NM11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a native human p300. The KAT2B (lysine acetyltransferase 2B) gene encodes a p300/CBP (CREB binding protein)-associated factor, which belongs to the family of GNAT (GCN5 (general control nonderepressible 5)-related N-acetyltransferase). |
| Immunogen: | native human p300. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |