Monoclonal Anti-PSF antibody produced in mouse
SIGMA/P2860 - clone B92, purified from hybridoma cell culture
Synonym: Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
MDL Number: MFCD03457573
Product Type: Chemical
Western Blotting JOURNAL CITATION: Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells. By: Chi, F., Bo, T., et al. in PLoS One, 2012. PubMed ID: 22536447 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0035862), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells. By: Chi, F., Bo, T., et al. in PLoS One, 2012. PubMed ID: 22536447 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0035862), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-PSF Clone: B92 (Cat. No. P2860). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. HepG2 3. A431 4. HUVEC 5. NCI-H1299 6. WiDr 7. Hepa 1-6 8. MM3MG
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | B92, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| radioimmunoassay: suitable using cells | |
| western blot: 2-4 μg/mL using whole extract of cultured HeLa cells |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) Immunoprecipitation (1 paper) |
| Application: | Monoclonal Anti-PSF antibody produced in mouse has been used in: • immunofluorescence • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues. |
| Immunogen: | lysate of mouse bone marrow-derived stromal cell line. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |