Anti-Cytokeratin pan antibody, Mouse monoclonal
SIGMA/P2871 - clone C-11, purified from hybridoma cell culture
Synonym: Anti-CK-4; Anti-CK4; Anti-CYK4; Anti-K4; Anti-WSN1
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human skin sections with 15 μg/mL Monoclonal Anti-Cytokeratin, pan Clone: C-11 (Cat. No. P2871) followed by Goat Anti-Mouse IgG (Fab)-FITC (Cat. No. F5262).
Immunofluorescence PTK2-Marsupial Kidney cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 30 μg/mL Monoclonal Anti-Cytokeratin, pan antibody produced in Mouse (Cat. No. P2871) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C-11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin | |
| immunohistochemistry (frozen sections): suitable | |
| microarray: suitable | |
| western blot: suitable |
| Application: | Monoclonal Anti-Cytokeratin pan antibody has been used in immunoblotting, dot blot, immunohistochemistry, immunocytochemistry, immunofluorescence, immunostaining, detecting micro-metastases in lymph nodes, bone marrow, and other tissues and for determining the origin of poorly differentiated tumors. |
| Biochem/physiol Actions: | Cytokeratin plays a major role in observing carcinomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cytokeratin, pan (mouse IgG1 isotype) is derived from the C-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cytokeratin is a member of the intermediate filaments family. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |