Monoclonal Anti-Protein A antibody produced in mouse
SIGMA/P2921 - clone SPA-27, ascites fluid
Synonym: Monoclonal Anti-Protein A
Product Type: Chemical
ELISA Monoclonal Anti-Protein A antibody produced in Mouse, Clone: SPA-27 (Cat. No. P2921), followed by 1:500 Anti-Mouse IgG (H+L), F(ab′)2 fragment, highly cross adsorbed-Peroxidase antibody produced in Goat (Cat. No. SAB3701122), was serially diluted to detect Protein A by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SPA-27, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: 1:20,000 | |
| western blot: suitable |
| Application: | Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses. |
| Biochem/physiol Actions: | Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers. |
| Biochem/physiol Actions: | Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain. |
| Immunogen: | protein A from Cowan I strain of Staphylococcus aureus. |
| Physical form: | Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |