Endoproteinase Glu-C from Staphylococcus aureus V8
SIGMA/P2922 - Type XVII-B, lyophilized powder, 500-1,000 units/mg solid
Synonym: V8 Protease
CAS Number: 66676-43-5
MDL Number: MFCD01324998
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 29 kDa |
| purified by | chromatography |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | 500-1,000 units/mg solid |
| storage temp. | −20°C |
| type | Type XVII-B |
| Application: | Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract. |
| Application: | Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments. |
| Application: | It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping. |
| Biochem/physiol Actions: | Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate. |
| Other Notes: | One unit will hydrolyze 1 μmole of N-t-Boc- |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42/43 |
| Safety Statements | 22-26-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: 500-1,000 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.19 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |