PIPES sodium salt

SIGMA/P2949 - ≥99% (titration)

Synonym: Na-PIPES; Piperazine-1,4-diethanesulfonate sodium salt; 1,4-Piperazinediethanesulfonic acid sodium salt; Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt

CAS Number: 10010-67-0
Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₆S₂
Molecular Weight: 324.35
EC Number: 233-005-3
MDL Number: MFCD00065472
Linear Formula: C₈H₁₇N₂O₆S₂Na
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P2949-25G	25 g	$61.50 1/EA	Add To Favorites
45-P2949-100G	100 g	$195.00 1/EA	Add To Favorites
45-P2949-500G	500 g	$750.00 1/EA	Add To Favorites

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This image depicts SKU: P2949-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing
assay	≥99% (titration)
form	crystalline powder
InChI	1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1
InChI key	OGGAIRCLBMGXCZ-UHFFFAOYSA-M
pH	4.5-4.7
pKa (25 °C)	6.8
Quality Level	200
SMILES string	[Na+].OS(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
solubility	0.1 M NaOH: 250 mg/mL, clear, colorless
storage temp.	room temp
useful pH range	6.1-7.5

Application:	PIPES sodium salt buffer has been used: • to prepare BRB80 buffer for imaging dynamic microtubules and single kinesin molecules through fluorescence microscopy • as a component of PEM fixation solution • to reconstitute red blood cells (RBCs) to study the effect of pH on 5-coordinate alpha-nitrosyl-hemoglobin (HbNO) stability
Features and Benefits:	• Non-toxic • Easy to use • Thermally stable • Easy to handle and store
General description:	1, 4-piperazinediethanesulfonic acid (PIPES) is a zwitterionic buffer that belongs to the group of the ethanesulfonic acid buffer series, first introduced by Good et al., It is a non-toxic buffer used in cell culture and tissue fixation for electron microscopy and other biological applications. PIPES don’t interact with metal ions. Since its pK_a at 37°C is near physiological pH, it has been used as a buffering substance in biological, biochemical, and environmental studies. PIPES is developed to meet certain criteria: midrange pK_a, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK_a with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Buffers can be prepared by adding a solution of the base to PIPES-free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.
Packaging:	25, 100, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (titration)
Storage Temp.	room temp
UNSPSC	12161700

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