Pertussis toxin from Bordetella pertussis
SIGMA/P2980 - buffered aqueous glycerol solution
Synonym: Histamine-
CAS Number: 70323-44-3
MDL Number: MFCD00131841
Product Type: Chemical
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| description | aseptically packaged |
| form | buffered aqueous glycerol solution |
| InChI | 1S/C26H52O2/c1-3-4-5-6-7- |
| InChI key | WOPKHAQDUMDJIY-UHFFFAOYSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | O(C)C(=O)CCCCCCCCCCCCCCCC |
| storage temp. | −20°C |
| Application: | Inactive toxin form released from B. pertussis. |
| Application: | Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model; as an inhibitor of Gαi signaling |
| Application: | The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. |
| Biochem/physiol Actions: | Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. |
| Biochem/physiol Actions: | Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. |
| Features and Benefits: | This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm . |
| General description: | Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP. |
| Physical form: | Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |