Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)
SIGMA/P302-05
Product Type: Product-on-demand
Cell Applications : Porcine Pulmonary Artery Endothelial Cells, PPAEC
| biological source | Porcine pulmonary artery (Normal healthy) |
| form | solid |
| growth mode | Adherent |
| karyotype | 2n = 38 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Endothelial |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | diabetes; cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs |
| Biochem/physiol Actions: | Artery |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013). |
| Other Notes: | Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO |
| Preparation Note: | • 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO • Can be cultured at least 16 doublings |
| Preparation Note: | Please refer to the PPAEC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |