Pectolyase from Aspergillus japonicus
SIGMA/P3026 - lyophilized powder, ≥0.3 units/mg solid
Synonym: Pectolytic enzyme
MDL Number: MFCD00131813
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3026-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3026-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3026-250MG
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| biological source | Aspergillus sp. (A. japonicus) |
| form | lyophilized powder |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 50 kDa |
| Quality Level | 200 |
| specific activity | ≥0.3 units/mg solid |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| Application: | Pectolyase from Aspergillus japonicus has been used: • in the lysis of chlorella cells for protoplast generation • as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation • to induce reorganization of Arabidopsis root cap cell architecture |
| Application: | Used in plant protoplast preparation to digest cell wall prior to organelle isolation. |
| Application: | Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis. |
| Biochem/physiol Actions: | Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-ga |
| Biochem/physiol Actions: | Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research . Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8. |
| Other Notes: | One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C. |
| Other Notes: | Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1 g in poly bottle |
| Packaging: | 100, 250 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.3 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.15 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |