Platelet-Derived Growth Factor-AA human
SIGMA/P3076 - PDGF-AA, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: PDGF−AA
MDL Number: MFCD00081862
Product Type: Chemical
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| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg |
| mol wt | 28.5 kDa (containing 250 amino acid residues) |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | 1.0-3.0 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04085  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells. |
| Biochem/physiol Actions: | Cell division and relocalization of pulmonary artery smooth muscle cells and fibroblasts is known to be triggered by platelet-derived growth factor (PDGF). Due to its proliferatory function, it is associated with fibroproliferative disorders such as atherosclerosis, pulmonary arterial hypertension and lung fibrosis. PDGF exists both in homo and heterodimeric forms and stimulates α and β-cell surface receptors. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signalling and is known to promote tumor angiogenesis in vivo. |
| General description: | Platelet-derived growth factor (PDGF) is composed two polypeptide subunits of A and B chain. PDGF is produced by a number of cells including smooth muscle cells, endothelial cells and macrophages. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors. |
| Other Notes: | Homodimer of the A chain of human PDGF. |
| Physical form: | Sterile-filtered through a 0.2 μm filter. Lyophilized with 10 mM acetic acid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |