Monoclonal Anti-Parvalbumin antibody produced in mouse
SIGMA/P3088 - clone PARV-19, ascites fluid
Synonym: Parvalbumin Antibody; Parvalbumin Antibody - Monoclonal Anti-Parvalbumin antibody produced in mouse
MDL Number: MFCD00164711
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus. By: Kinjo, E. R., Higa, G. S., et al. in PLoS One, 2014. PubMed ID: 25299405 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0109149), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: The role of egr1 in early zebrafish retinogenesis. By: Zhang, L., Cho, J., et al. in PLoS One, 2013. PubMed ID: 23405257 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0056108), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus. By: Kinjo, E. R., Higa, G. S., et al. in PLoS One, 2014. PubMed ID: 25299405 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0109149), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Monoclonal Anti-Parvalbumin: Cat. No. P3088: Immunoperoxidase parvalbumin staining of a coronal section of the Rat hippocampal formation (low power magnification) The inset shows a detail of the dentate gyrus and CA3 areas. Submitted by Dr C. De Cabo De La Vega, Albacete University.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat cerebellum sections with 1:2,000 Monoclonal Anti-Parvalbumin, Clone: PARV-19 (Cat. No. P3088) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat cerebellum sections with 1:2,000 Monoclonal Anti-Parvalbumin, Clone: PARV-19 (Cat. No. P3088) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PARV-19, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 12 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | fish, frog, rabbit, canine, feline, bovine, pig, human, goat, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using formalin-fixed, paraffin-embedded rat tongue or cerebellum sections |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P20472  |
| Application: | Monoclonal Anti-Parvalbumin antibody produced in mouse has been used for immunohistochemistry, immunocytochemistry, and immunofluorescence. |
| Biochem/physiol Actions: | PVALB (parvalbumin) protein modulates relaxation in fast contracting muscles, where it localizes to type II (fast-twitch) mammalian skeletal muscle fibres in small mammals. This protein is a crucial regulator of neuronal intracellular calcium dynamics. PVALB protein maintains synaptic transmission during bursts, where it prevents presynaptic Ca2+ accumulation and hence the harmful effects of excess presynaptic Ca2+ levels. It also maintains synaptic transmission by allowing for linear information processing post-synaptically by preventing cumulative neuronal facilitation. PVALB (parvalbumin) expression serves as marker for differentiating tumors as benign and malignant, in relation with thyroid lesions. Decreased expression of parvalbumin positive interneurons in rat model is associated with schizophrenia, and is known to disturb neuronal activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PVALB (parvalbumin) is a classical small member of the low molecular weight, acidic protein family known as calcium binding proteins (CaBPs) EF-hand superfamily. This protein shows a restricted pattern of expression in brain and muscle. It is a cytoplasmic high affinity CaBP. Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the PARV-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Parvalbumin is a calcium-binding protein. |
| Immunogen: | Frog muscle parvalbumin. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Liquid |
| Specificity: | Recognizes parvalbumin in a Ca2+ ion-dependent manner. Does not react with other members of the EF-hand family such as calmodulin, intestinal calcium-binding protein, S100A2 (S100L), S100A6 (calcyclin), the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b). |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |