Monoclonal Anti-p115/TAP antibody produced in mouse
SIGMA/P3118 - clone 5D6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Transcytosis Associated Protein
MDL Number: MFCD03792426
Product Type: Chemical
Western Blotting Rat liver extract were separated on SDS-PAGE and probed with Monoclonal Anti-p115/TAP antibody produced in Mouse, Clone: 5D6 (Cat. No. P3118). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:1,000 antibody 4. 1:2,000 antibody 5. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5D6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver | |
| UniProt accession no. | P41542  |
| Application: | Monoclonal Anti-p115/TAP antibody produced in mouse may be used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa). |
| Biochem/physiol Actions: | p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra−Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitiv |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain. |
| Immunogen: | purified rat p115/TAP. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |