Platelet-Derived Growth Factor-BB human
SIGMA/P3201 - PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: PDGF-BB
MDL Number: MFCD00213023
Product Type: Chemical
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| assay | ≥98% (HPLC and SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | ~25 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | 1.0-3.0 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01127  |
| Analysis Note: | The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts. |
| Application: | Platelet-Derived Growth Factor-BB human has been used : • As a growth supplement in proximal tubule and glomerular explant cell culture • In three-dimensional scaffold preparation • To study the effect of brivanib on growth factor signaling pathways • In transwell migration assay |
| Biochem/physiol Actions: | Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding. PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration. |
| General description: | Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms. |
| Other Notes: | Dimer of the B chain of human PDGF. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |