Phosphodiesterase I from Crotalus adamanteus venom
SIGMA/P3243 - vial of ≥100 units, Purified
Synonym: 5′-Exonuclease; Oligonucleate 5′-nucleotidohydrolase
CAS Number: 9025-82-5
EC Number: 232-806-5
MDL Number: MFCD00131883
Product Type: Chemical
Catalyzes the exonucleolytic cleavage in the 3′- to 5′-direction to yield nucleoside 5′-phosphates.
| biological source | Crotalus adamanteus venom |
| form | solid |
| packaging | vial of ≥100 units |
| quality | Purified |
| Quality Level | 200  |
| specific activity | ≥20.0 unit/mg solid |
| storage temp. | −20°C |
| Application: | Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides. |
| Biochem/physiol Actions: | Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-spec |
| Other Notes: | One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9 |
| Preparation Note: | For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. |
| Preparation Note: | Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20.0 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |