Platelet-Derived Growth Factor-AB human
SIGMA/P3326 - PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: PDGF-AB
MDL Number: MFCD00213024
Product Type: Chemical
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| assay | ≥97% (SDS-PAGE and N-terminal analysis) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | 27-31 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | 10-100 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04085, P01127  |
| Analysis Note: | The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts. |
| Application: | Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay. |
| Biochem/physiol Actions: | Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis. PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes. |
| General description: | Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3. |
| Other Notes: | Heterodimer of the A and B chains of PDGF disulfide linked. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE and N-terminal analysis) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |