Papain from papaya latex
SIGMA/P3375 - crude powder, 1.5-10 units/mg solid
Synonym: Papainase
CAS Number: 9001-73-4
EC Number: 232-627-2
MDL Number: MFCD00131791
Product Type: Chemical
Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Papain is used routinely for the preparation of Fab fragments from IgG. IgM may also be digested with papain resulting in high yields of homogeneous Fab preparations.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3375-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3375-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3375-1KG
| form | crude powder |
| mol wt | 23.4 kDa |
| Quality Level | 200  |
| specific activity | 1.5-10 units/mg solid |
| storage temp. | 2-8°C |
| Analysis Note: | Not standardized with lactose or other adulterants. |
| Application: | Papain from papaya latex has been used: • in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder • in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules • as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents |
| Application: | Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate. |
| Application: | Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. |
| Biochem/physiol Actions: | A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. |
| Biochem/physiol Actions: | Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. . The pH optimum is 6.0-7.0 . Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. |
| Other Notes: | One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginin |
| Other Notes: | View more information on papain at www.sigma-aldrich.com/enz. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 25, 100 g in poly bottle |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1.5-10 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.22.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |