Phosphoglucomutase from rabbit muscle
SIGMA/P3397 - ammonium sulfate suspension, ≥100 units/mg protein
Synonym: PGM; α-D-Glucose-1,6-bisphosphatase; α-D-Glucose-1-phosphate phosphotransferase
CAS Number: 9001-81-4
MDL Number: MFCD00131886
Product Type: Chemical
Unit Definition: One unit of phosphoglucomutase will convert 1.0 μmole of α-D-Glucose-1-Phosphate to α-D-Glucose-6-Phosphate per minute at pH 7.4 at 30 °C. The continuous spectrophotometric rate detemination (A340, Light path = 1 cm) is based on the above reactions.
| biological source | rabbit muscle |
| color | white to light yellow |
| description | ammonium sulfate suspension, >=100 units/mg protein |
| foreign activity | lactic dehydrogenase ≤0.5% |
| phosphoglucose isomerase ≤0.01% | |
| pyruvate kinase ≤0.05% | |
| form | ammonium sulfate suspension |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | ≥100 units/mg protein |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| Analysis Note: | Protein determined by biuret. |
| Application: | Phosphoglycomutase has been used: • to study glycogenesis and phosphoglycomutase deficiency in humans • in phosphoglucomutase assays to study metabolic regulation • for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells • in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3) • in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP+)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi) |
| Biochem/physiol Actions: | Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P). |
| General description: | Research area: Cell Signaling Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues. |
| Other Notes: | One unit will convert 1.0 μmole of α- |
| Packaging: | 1000 units in glass bottle |
| Physical form: | Crystalline suspension in 3.2 M (NH4)2SO4, pH 6.0 containing 0.01% EDTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 5.4.2.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |