Monoclonal Anti-Phosphoserine antibody produced in mouse
SIGMA/P3430 - clone PSR-45, ascites fluid
Synonym: Monoclonal Anti-Phosphoserine; Phospho Ser; Phospho serine; Phospho−Ser; Phospho−serine
Product Type: Chemical
Monoclonal Anti-Phosphoserine, Clone PSR-45 (P3430)
Immunoblot
Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine (Product No. P3430). The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate (Product No. B7151), ExtrAvidin, Peroxidase Conjugate (Product No. E2886) and AEC substrate.
Lane A: Antibody dilution 1:1,000
Lane B: Negative control (only secondary antibody)
Immunoblot
Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine (Product No. P3430). The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate (Product No. B7151), ExtrAvidin, Peroxidase Conjugate (Product No. E2886) and AEC substrate.
Lane A: Antibody dilution 1:1,000
Lane B: Negative control (only secondary antibody)
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PSR-45, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:4,000 |
| western blot: 1:500-1:1,000 |
| Application: | Anti-Phosphoserine antibody may be used for indirect ELISA at a working dilution of 1:4000. For immunoblotting using rat brain cortex extracts, a working dilution of 1:500-1:1100 may be used. The antibody was used for immunoblotting to detect proteins phosphorylated at serine in stem extracts of Arabidopsis thalliana at a working dilution of 1:250. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) Western blotting following immunoprecipitation (1 paper) |
| Application: | Monoclonal Anti-Phosphoserine antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site. |
| Biochem/physiol Actions: | Phosphoserine (PS) can be used as a template to detect cancer antigen 125 (CA 125). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |