Monoclonal Anti-PTEN antibody produced in mouse
SIGMA/P3487 - ~1 mg/mL, purified immunoglobulin, clone PTN-18, buffered aqueous solution
Synonym: Anti-Mutated in multiple advanced cancers 1; Anti-PTEN1; Anti-PTENbeta; Anti-Phosphatase and tensin homolog; Anti-TEP1
MDL Number: MFCD02097285
Product Type: Chemical
Immunoprecipitation Monoclonal Anti-PTEN, Clone: PTN-18 (Cat. No. P3487) was used to immunoprecipitate PTEN from HeLa (lanes 1-2) and HT-29 (lanes 3-4) whole cell lysate. Detection antibody: Rabbit Anti-PTEN (Cat. No. P7482) followed by Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 10 μg antibody 2. Negative control: without antibody 3. 10 μg antibody 4. Negative control: 10 μg nonrelevant antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PTN-18, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using 4% paraformaldehyde-0.5% Triton X-100 fixation |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 5-10 μg/mL using whole extract of rat brain | |
| UniProt accession no. | P60484  |
| Biochem/physiol Actions: | The epitope recognized by the antibody resides within amino acids 386-403 of rat PTEN. |
| Biochem/physiol Actions: | The PTEN (phosphatase and tensin homolog) gene encodes a encodes a dual-specificity phosphatase that functions as a tumor suppressor by inhibiting phosphoinositide-3-kinase |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The PTEN (phosphatase and tensin homolog) gene is mapped to human chromosome 10q23. It shares sequence homology with protein tyrosine phosphatases and the cytoskeletal protein tensin. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of rat PTEN (amino acids 386-403). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |