Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)
SIGMA/P354-05
Product Type: Product-on-demand
Cell Applications : Porcine Aortic Smooth Muscle Cells, PAOSMC (left). PAOSMC immunolabeled for smooth muscle-specific alpha-actin (ABC method, right).
| biological source | Porcine aorta (normal) |
| form | solid |
| growth mode | Adherent |
| karyotype | 2n = 38 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | smooth muscle |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation |
| Biochem/physiol Actions: | Aorta |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008). Characterization: positive for smooth muscle cell specific alpha-actin expression. |
| Other Notes: | Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO |
| Preparation Note: | • 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO • Can be cultured at least 16 doublings |
| Preparation Note: | Please refer to the PAOSMC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |