Monoclonal Anti-Phosphothreonine antibody produced in mouse
SIGMA/P3555 - clone PTR-8, ascites fluid
Synonym: Monoclonal Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human prostate sections with 1:200 Monoclonal Anti-Phosphothreonine Clone: PTR-8 (Cat. No. P3555) using biotinExtrAvidin®-Peroxidase.
Immunofluorescence A549 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:100 Monoclonal Anti-Phosphothreonine, Clone: PTR-8 (Cat. No. P3555). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Lysate from HS68 cells was separated on SDS-PAGE and probed with Monoclonal Anti-Phosphothreonine, Clone: PTR-8 (Cat. No. P3555). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution: 1:50 2. Antibody dilution: 1:100
Flow Cytometry 1×106 A549 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 1:100 Monoclonal Anti-Phosphothreonine, Clone: PTR-8 (Cat. No. P3555) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PTR-8, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:4,000 |
| western blot: 1:50 |
| Application: | Monoclonal and polyclonal antibodies directed against phosphorylated residues may be useful as analytical and preparative tools, by enabling the identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins. Antibodies can be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or even whole animals. Mouse monoclonal clone PTR-8 anti-phosphothreonine antibody may be used for the localization of phosphorylated threonine using various immunochemical assays such as ELISA, dot blot, and immunoblotting. Due to steric hindrance of the recognition site, this antibody may not recognize certain proteins known to contain phosphorylated threonine. |
| Application: | Monoclonal Anti-Phosphothreonine antibody produced in mouse has been used in:immunoblotting, enzyme linked immuno sorbent assay (ELISA) ,dot blot. |
| Application: | Mouse monoclonal clone PTR-8 anti-Phosphothreonine antibody reacts with phosphorylated threonine both as a free amino acid or when conjugated to carriers such as BSA or KLH, using ELISA and dot blot. It does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP or ATP. |
| Biochem/physiol Actions: | Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue. However, the abundance of phosphorylated cellular proteins increases tenfold following various activation processes, which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-Tyr/p-Ser/p-Thr). Many different mitogenic systems, such as the EGF, PDGF and insulin receptor systems, contain Tyr/Ser/Thr kinase domains that autophosphorylate specific Tyr/Ser/Thr residues upon binding of their ligands. T cell antigen receptor complex or receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated Tyr/Ser/Thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects the properties of these proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containi |
| General description: | Monoclonal Anti-Phosphothreonine (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | phosphothreonine conjugated to keyhole limpet hemocyanin (KLH). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |