Phosphatase, Acid from wheat germ
SIGMA/P3627 - ≥0.4 unit/mg solid
Synonym: (Orthophosphoric-
CAS Number: 9001-77-8
EC Number: 232-630-9
MDL Number: MFCD00131847
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3627-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3627-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3627-1G
| form | powder |
| InChI | 1S/C6H10O2/c1-3-4-8-5-6(2 |
| InChI key | GZCWLCBFPRFLKL-UHFFFAOYSA |
| mol wt | 58 kDa by gel filtration |
| Quality Level | 300  |
| SMILES string | O(CC(O)C)CC#C |
| specific activity | ≥0.4 unit/mg solid |
| storage temp. | −20°C |
| Analysis Note: | Contains lipase |
| Application: | Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining. |
| Biochem/physiol Actions: | Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C. |
| Packaging: | 1, 5, 10 g in poly bottle |
| Preparation Note: | Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.4 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |