Anti-Protein A antibody produced in rabbit
SIGMA/P3775 - fractionated antiserum, lyophilized powder
Synonym: Anti-Protein A
MDL Number: MFCD00164723
Product Type: Chemical
ELISA Anti-Protein A antibody produced in Rabbit (Cat. No. P3775), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| packaging | vial of 2 mL lyophilized antiserum |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:100,000 |
| Application: | Anti-Protein A antibody produced in rabbit has been used in immunoblotting. |
| Application: | Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000. |
| Biochem/physiol Actions: | Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus. |
| General description: | The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation. |
| Immunogen: | Protein A from Staphylococcus aureus |
| Physical form: | Lyophilized from 0.01M phosphate buffered saline, pH 7.2 |
| Preparation Note: | Reconstitute with 2 ml deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |