Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA
SIGMA/P3803 - Molecular Biology
Synonym: DNA extraction
Product Type: Chemical
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| application(s) | agriculture |
| assay | ≥99.0% |
| form | liquid |
| grade | Molecular Biology |
| pH | 6.5-6.9(pH of phenolic phase) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids. |
| Disclaimer: | If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary. |
| Other Notes: | For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH. |
| Other Notes: | This product has a shelf life of two years. |
| Packaging: | 100, 400 mL in glass bottle |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 - H312 + H332 - H314 - H336 - H341 - H351 - H361d - H372 - H373 - H411 |
| Precautionary statements | P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 63-23/24/25-34-40-48/20/21/22-68 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 2810 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12190000 |